Oral Contraceptive Use May Modulate Global Genomic DNA Methylation and Promoter Methylation of APC1 and ESR1
نویسندگان
چکیده
Background: There are challenging reports in the public health sphere regarding associations between oral contraceptive (OC) use and cancer risk. Methods: To evaluate possible effects of OCs on cancer susceptibility, we quantified of global 5-methyl cytosine (5-mC) levels and assessed methylation patterns of CpG islands of two key tumor suppressor genes, APC1 and ESR1, in serum of users by enzyme-linked immunosorbent assay and methylation specific PCR methods, respectively. Results: Our results indicated that OCs significantly decrease the level of global DNA methylation in users relative to control non-users. However, our data revealed no significant differences between CpG island methylation patterns for ESR1 and APC1 in healthy control and OC-treated women. However, we did find a trend for hypermethylation of both tumor suppressor genes in OC users. Conclusion: Our data suggest that the level of 5-mC but not individual CpG island patterns is significantly influenced by OCs in our cross-section of adult users.
منابع مشابه
Evaluation of epigenetic changes of liver tissue induced by oral administration of Titanium dioxide nanoparticles and possible protective role of Nigella Sativa oil, in adult male albino rats
Objective (s): Titanium dioxide nanoparticles (TiO2 NPs) have been recognized as biologically inert material and have been used in a multitude of applications. Nevertheless, the negative impact on the human health is not yet well understood. Aim of the work: The study attempted to evaluate the epigenetic changes of the genome, in the form of DNA methylation in liver tissue samples, resulting fr...
متن کاملA preliminary study of the association between the ABCA1 gene promoter DNA methylation and coronary artery disease risk
Coronary artery disease (CAD) is a common health problem in Iranian population. ATP binding cassette transporter A1 (ABCA1) plays central role in the efflux of the cholesterol from peripheral tissues back to liver. Inactivation of ABCA1 by epigenetic change such as DNA methylation may contribute to the development of CAD. The present study investigated the association between promoter DNA methy...
متن کاملComparing Oprm1 Gene Promoter Methylation in the Lymphocytes of Male Rats Addicted to Nicotine, Morphine,Methadone, and Buprenorphine
Background: Addiction is a polygenic disorder caused by genetic and environmental factors. The opioid material can act as an epigenetic element, like DNA methylation. The present study aimed to examine the effect of epigenetic drugs such as nicotine, morphine, methadone, and buprenorphine on the methylation of two CpG sites in promoter of Oprm1 gene in male Wistar rats. Materials & Methods: In...
متن کاملStudy of promoter CpG island hypermethylation of cyclindependent kinase inhibitor gene p21waf1/cip1 on some breast carcinoma cell lines
The p21 belongs to the CIP/KIP family of CDK inhibitors involved in cell cycle arrest at specific stages of the cell cycle progression. DNA methylation is the best studied epigenetic mark that have been evidently associated to chromatin condensation, and repression of gene transcription. The CpG island hypermethylation in promoter region of certain genes occurs in cancer cells and affects tumor...
متن کاملP-13: Comparison of Sperm Quality, Oxidative Stress, DNA Fragmentation, Protamine Deficiency, and DNA Methylation in Varicocele and Fertile Individuals
s:1993:"Background: There are many approaches that gene expression is controlled in eukaryotes. DNA methylation is one of several epigenetic mechanisms that cells use to control gene expression and lock genes in the "off" position. In addition, sperm DNA damage can correlate with DNA methylation defect. There is evidence that sperm of infertile men contain more DNA damage than fertile men and t...
متن کامل